Biotech Training And Research Centre In Lucknow | Experiome

Immunoblotting Immunoblotting, often known as western blotting, is a technique for separating proteins by molecular weight. Two gels are created in this technique: a stacking gel pH (6.8) and a separating gel pH (8.8). The samples are put onto an acidic stacking gel, which separates proteins poorly but allows them to form narrow, highly defined bands. Smaller proteins travel quicker than larger protein molecules on a separating or resolving gel used to separate proteins based on their size. When voltage is supplied to the protein before loading, it is denatured and has a negative charge that travels towards the positive electrode. After the protein separation, the gel is sliced and inserted in a sandwich of fibre pad and filter sheets with a membrane (PVDF or nitrocellulose) and placing it in the tank with the transfer buffer with overnight transfer of protein to the membrane. After the protein has been transferred to the membrane, it is blocked by blocking solution to prevent antibodi...

Next Generation Sequencing (NGS) Next generation sequencing also known as high-throughput sequencing as it can sequence many DNA strands at the same time. This technology is used to study genetic variations associated with diseases by determining the sequence of DNA or RNA. Methods of Next generation sequencing are as follows:   1. Massively Parallel Signature Sequencing This technology uses type II restriction endonuclease, a mixture of adapters with all possible overhangs which is annealed to the target sequence, leading in a single adapter with a perfect complimentary overhang being ligated. Since every adapter has its own label, the overhangs are recognised immediately after ligation. The number of oligonucleotide tags is 100 times greater than the number of template samples, ensuring that each template sample is conjugated to a unique tag. Signatures are generated by monitoring successful adapter ligations onto a surface of microbeads in a flow cell. This procedure uses ...

Biotech Training in Lucknow |  Experiome Why Biotechnology trainingis important and relevant to the students of PG and UG? Biotechnology consists of altering biological function by adding or removing genes from another organism. This course isintendedfor students with abackgroundin biology and nodding acquaintance in maths and chemistry till class 12. After graduation it is necessary to pass national level examinations to be part of academics. Apart of this biotechnology has a significant impact on a variety of industries, including biomedical, agriculture, health care, farming, food processing, the environment, chemicals, and textiles. Considering the situation, biotechnology has grown in popularity over time, and in the not-too-distant future, it will reach the skies. So, what really is the significance of training? It is critical for students to have hands-on training and understanding in the area. According to the current situation, freshers do not receive adequate hands-o...

Experiome | Biotechnology Projects for Undergraduate and Postgraduate Students CRISPR-Cas use to meet the need of Vitamin A Nutrition is essential for healthy growth and development. Macronutrients or micronutrients are the two types of nutrients. Requirements of macronutrients including fats, proteins, and carbohydrates are relatively higher to that of micronutrients including several vitamins and minerals. In comparison, if the amount of micronutrients in the diet is low, it has an impact on the child's physical and mental development.  Vitamin A is a lipid-soluble vitamin that is required for cell growth, metabolism, immunological function, eyesight, and reproduction.It is absorbed in the duodenum and stored in hepatic stellate cells, as well as in adipose tissue and the pancreas in small amounts. Vitamin A is found naturally in dark leafy greens, orange-colored vegetables, milk products, liver, and fish. Vitamin A intake should be around 200 micrograms per day for children...