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  Guest Speaker: Dr Chandra Prakash Chaturvedi, PhD Associate Professor, Department of Hematology, SGPGIMS, Lucknow Title of the talk: Fundamentals of Recombinant DNA Technology Life Science Training Centre in Lucknow | Biotech Research Centre in Lucknow | Summer Internship for Biotechnology Students in Lucknow | Biotech Training in Lucknow

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  Polymerase Chain Reaction (PCR) An American biochemist named Kary B. Mullis invented PCR in 1983. Prior to the advent of PCR, the process of amplifying, or making copies of, recombinant DNA fragments was time- consuming and labor-intensive. The polymerase chain reaction (PCR), sometimes known as "molecular photocopying," is a simple and inexpensive process for amplifying, or copying, small pieces of DNA. Because molecular and genetic analyses require large amounts of DNA, studies of isolated fragments of DNA are nearly impossible without PCR amplification. To amplify the DNA segment in PCR, the template DNA is heated for its denaturation. Taq polymerase is an enzyme that creates two new strands of DNA using the previous strands as templates. This process causes the original DNA to be duplicated, with each new molecule comprising one old and one new strand of DNA. Then, for each of these strands, two new copies can be made, and so on. Denaturing and synthesizing new

 Reverse Breeding Reverse breeding is used to establish parental lines for desired hybrid lines (not genetically modified). Homozygous lines are produced from heterozygous plants to achieve this. This is accomplished by introducing a gene that prevents recombination during meiosis into the heterozygous line (the hybrid). As a result, the genetically engineered plant's haploid gametes have completely non-recombined chromosomes. After that, the gametes can be used to create plants. Only non-genetically modified plants are used, and those that have the transgenic DNA are removed. Genes that aid recombination in meiosis can be suppressed via RNAi. Meiotic recombination involves a number of genes. Biotechnology Training Program in Lucknow Asy1 or sds, which ensure that homologous chromosomes pair in the first phase of meiosis, are genes that can be silenced. Furthermore, the spo11-1gene, which is responsible for the occurrence of double-strand breaks during recombination, can be tu

  Ethics and Requirements of Plant Tissue Culture Laboratory Plant tissue culture generally refers to   in vitro culture of all plant parts under sterile conditions. Basic facilities are must in the laboratory where tissue culture technology is to be performed. This includes general cleaning and media preparation, sterilization, storage, sterile transfer, observation / data acquisition, and environmentally controlled incubator or culture room areas. The most important work area is the Cultural Transfer Office, where   core activities take place. Plant tissue culture should be cultured for a specified period of time under well-controlled temperature, humidity, airflow, and light quality. Plant culture techniques require a variety of organic and inorganic chemicals to prepare the   culture medium. The growing room is an equally important area where plant cultures are maintained under controlled environmental conditions for optimal growth. After in vitro culture of plants they are transfe